Frzb-1 Is a Secreted Antagonist of Wnt Signaling Expressed in the Spemann Organizer
نویسندگان
چکیده
Frzb-1 is a secreted protein containing a domain similar to the putative Wnt-binding region of the frizzled family of transmembrane receptors. Frzb-1 is widely expressed in adult mammalian tissues. In the Xenopus gastrula, it is expressed and regulated as a typical Spemann organizer component. Injection of frzb-1 mRNA blocks expression of XMyoD mRNA and leads to embryos with enlarged heads and shortened trunks. Frzb-1 antagonizes the effects of Xwnt-8 ectopic expression in a non-cell-autonomous manner. Cultured cells transfected with a membrane-tethered form of Wnt-1 bind epitope-tagged Frzb-1 in the 10(-10) M range. The results strengthen the view that the Spemann organizer is a source of secreted inhibitory factors.
منابع مشابه
Frzb, a Secreted Protein Expressed in the Spemann Organizer, Binds and Inhibits Wnt-8
We isolated a Xenopus homolog of Frzb, a newly described protein containing an amino-terminal Frizzled motif. It dorsalized Xenopus embryos and was expressed in the Spemann organizer during early gastrulation. Unlike Frizzled proteins, endogenous Frzb was soluble. Frzb was secretable and could act across cell boundaries. In several functional assays, Frzb antagonized Xwnt-8, a proposed ventrali...
متن کاملCell–cell signalling: Frog frizbees
The recent discovery that Frizzled proteins are receptor for Wnts has been quickly followed by the identification of a secreted protein, Frzb, that is related to Frizzled, expressed by the Spemann organizer in frog embryos and can bind to and antagonize Wnt developmental signalling molecules.
متن کاملThe agony column
It has recently been shown that a class of secreted proteins — the Dickkopfs — are released by the Spemann organizer of Xenopus and are potent antagonists of Wnt signaling [1]. Although it has been reported that the Dickkopf (Dkk) proteins contain two cysteine-rich domains, no direct functional or structural conclusions have been drawn from their sequences. The role of Wnt signaling in animal d...
متن کاملA colipase fold in the carboxy-terminal domain of the Wnt antagonists – the Dickkopfs
It has recently been shown that a class of secreted proteins — the Dickkopfs — are released by the Spemann organizer of Xenopus and are potent antagonists of Wnt signaling [1]. Although it has been reported that the Dickkopf (Dkk) proteins contain two cysteine-rich domains, no direct functional or structural conclusions have been drawn from their sequences. The role of Wnt signaling in animal d...
متن کاملProtein folding: Think globally, (inter)act locally
It has recently been shown that a class of secreted proteins — the Dickkopfs — are released by the Spemann organizer of Xenopus and are potent antagonists of Wnt signaling [1]. Although it has been reported that the Dickkopf (Dkk) proteins contain two cysteine-rich domains, no direct functional or structural conclusions have been drawn from their sequences. The role of Wnt signaling in animal d...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Cell
دوره 88 شماره
صفحات -
تاریخ انتشار 1997